Protein pattern analysis in tolerant and susceptible wheat cultivars under salinity stress conditions
Marouf KHALILI | Mohammad Reza NAGHAVI | Said YOUSEFZADEH
To investigate proteome pattern of wheat cultivars, young leaves were collected from tillering stage of seedlings two weeks after development of the salinity stress. The extraction of proteins from leaf tissue was done and two dimensional electrophoresis using IPG strips and SDS-PAGE in the control and salinity treatments were performed. In total, 198 and 203 protein spots were identified in tolerant (‘Moghan3’) and susceptible (‘Pishtaz’) cultivars respectively. Also, among these, spots number 21 and 22 were detected with significant IF in ‘Moghan3’ and ‘Pishtaz’ respectively. Two-stage mass spectrometry (MS/MS) was used to identify protein spots. Common identified proteins, including proteins involved in removal of oxidants, Calvin cycle proteins, proteins involved in light reaction of photosynthesis and proton transfer, and heat shock protein were identified on basis of the functional groups and their frequency. In total, ‘Moghan3’ maintained the stability of the structure and performance of carbon metabolism under stress better than susceptible cultivar. In addition, defense against oxidative stress induced by salinity stress was performed by 2-cys peroxiredoxin BAS1 and Cu-Zn SOD proteins that tolerant cultivar defended against oxidative stress better than the susceptible cultivar. The greatest strength of ‘Moghan3’ and major weakness in ‘Pishtaz’ are relying on the unique proteins formed under salinity stress for the removal of oxidants and to maintain the activity of the photosynthetic light reactions, respectively.
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