Construction of Microbial Consortium to Enhance Cellulose Degradation in Corn Straw during Composting

The improper treatment of crop straw not only leads to resource wastage but also adversely impacts the ecological environment. However, the application of microorganisms can accelerate the decomposition of crop straw and improve its utilization. In this study, cellulose-degrading microbial strains were isolated from naturally decayed corn straw and screened using Congo red staining, along with assessing variations in carboxymethyl cellulase (CMCase) activity, filter paper enzyme (FPase) activity and β-glucosidase (β-Gase) activity, as well as the degradation rate. The eight strains, namely <i>Neurospora intermedia</i> isolate 29 (A1), <i>Streptomyces</i> isolate FFJC33 (A2), <i>Gibberella moniliformis</i> isolate FKCB-009 (A3), <i>Fusarium fujikuroi</i> isolate EFS3(2) (A4), <i>Fusarium Fujikuroi</i> isolate FZ04 (A5), <i>Lysine bacillus macroides</i> strain LNHL43 (B1), <i>Bacillus subtilis</i> strain MPF30 (B2) and <i>Paenibacilli lautus</i> strain ALEB-P1 (C), were identified and selected for microbial strain consortium design based on their high activities of CMCase, FPase and β-Gase. The fungi, bacteria and actinomycete strains were combined without antagonistic effects on corn straw decomposition. The results showed the A2B2 combination had a significantly higher FPase at 55.44 U/mL and β-Gase at 25.73 U/mL than the other two strain combinations (<i>p</i> < 0.05). Additionally, the degradation rate of this combination was 40.33%, which was considerably higher than that of the other strains/consortia. The strain combination A4B2C also had superior enzyme activity, including CMCase with a value of 35.03 U/mL, FPase with a value of 63.59 U/mL and β-Gase with a value of 26.15 U/mL, which were significantly different to those of the other three strain combinations (<i>p</i> < 0.05). Furthermore, seven single microbial strains with high cellulase activities were selected to construct various microbial consortiums for in situ composting in order to evaluate their potential. Taken as a whole, the results of composting, including temperature, moisture content, pH, E4/E6 value and seed germination index, indicated that the microbial strain consortium consisting of <i>Neurospora intermediate</i> isolate 29, <i>Fusarium fujikuroi</i> isolate EFS3(2), <i>Fusarium fujikuroi</i> isolate FZ04, <i>Lysinibacillus macrolides</i>, <i>Lysinibacillus sphaericus</i>, <i>Bacillus subtilis</i> and <i>Paenibacillus lautus</i> was advantageous for corn straw decomposition and yielded high-quality compost. The screened flora was able to effectively degrade corn straw. This study provides a novel solution for the construction of a microbial consortium for the composting of corn straw.