In vitro isolation of a gamma ray induced mutant in chrysanthemum (Dendranthema grandiflora)
PRATIVA ANAND | VANLALRUATI VANLALRUATI | GUNJEET KUMAR | SURENDRA KUMAR
In the present investigation, an effort was made to develop an efficient ray floret regeneration protocol in order to isolate, purify and establish a novel gamma ray induced mutant in the form of brick red flowers in chrysanthemum (Dendranthema grandiflora Tzvelev.) cv. Tata Century (pink). Maximum survival (60.00%) and callus formation (56.60%) in minimum duration (10.20 days) were recorded when the ray florets were pre-treated with carbendazim (0.2%) + ridomil (0.2%) + 8-HQC (200 mg/l) for 2 h followed by surface sterilization with HgCl2 (0.1%) for a duration of 4 min and cultured on MS medium supplemented with BAP (4.0 mg/l) and NAA (1.0 mg/l). The maximum regeneration of microshoots (66.00%) from the ray floret induced callus was recorded on MS medium fortified with BAP (4.0 mg/l) + NAA (1 mg/l). MS medium supplemented with BAP (4.0 mg/l) + NAA (0.05 mg/l) + GA3 (0.1 mg/l) was found to be best for highest micro-shoot proliferation (80.00%). Highest rooting (87.60%) was induced after inoculating the microshoots individually on half-strength MS medium fortified with 0.5 mg/l NAA and 60 g/l sucrose. Successful acclimatization of in vitro raised plantlets was done in glass jar with polypropylene cap each filled with a mixture of sterilized cocopeat, soilrite and perlite (1:1:1 v/v) supplemented with half-strength MS inorganic salts. After 3-4 weeks of acclimatization the plantlets were successfully transferred to field conditions.
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