Effect of time and temperature of storage on the activity of the etanolic extracts of Lantana camara L. and Heliotropium indicum L. on Colletotrichum gloeosporioides
Rafael Alejandro Infante | Dorian Alcides Rodriguez | Maria Elena Sanabria | Dilcia Milagros Ulacio | Rosario Emperatriz Valera
The effect of time and temperature of storage of ethanolic extracts (EE) of Lantana camara and Heliotropium indicum on Colletotrichum gloeosporioides was investigated. The EE were stored at 8±2 and 26±2°C, during one year. Every 2 months they were diluted in potato dextrose agar (PDA) at 0; 0.5; 1 y 1.5% (v/v) concentrations, fungus disks were placed onto the media, mycelial growth (ICM) and sporulation (IE) inhibition were measured, as well as conidia germination. The EE stored at 8±2°C maintained their effectiveness on all variables during the 12 months storage, while those stored at 26±2°C started to lose their effectiveness at 6 to 8 months, ending up to 3% ICM, 4% IE and 18% conidia germination at 1.5% concentration. Results indicated that storage temperature and time are determinant for extracts effectiveness.
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