Effect of 2,4-D concentration in somatic embryos formation of cuban soybeans varieties
Idalmis Bermúdez-Caraballoso | Teresa Del Socorro Blanco | Jorge L. Pérez-Pérez | Lourdes R. García | Novisel Veitía | Raúl Collado | Damaris Torres | Carlos Romero
Developing new breeding programs of soybean crops needs an efficient systems for plants regeneration. Eventhough due this is impossible taking into account its genotype dependent condition. This study aimed to: determine the effect of 2,4-dichlorophenoxyacetic acid (2,4-D) concentration in the formation of somatic embryos in soybean Cuban varieties (Incasoy-1, Incasoy-24, Incasoy-27 and lncasoy-35). .mmature cotyledons were used as plant material. Three treatmentswere established according tothe concentrations of 2,4-D (30, 40 and 50 mg l1). The number of somatic embryos per immature cotyledon were evaluated after 30 days. The necessary concentration of 2,4-D to obtain the mayor formation of embryos depended on the genotypes (30 mg l1 for Incasoy-24; 40 mg l- for lncasoy-27 and lncasoy-35; 40 and 50 mg M for rIcasoy-1). Dependdng oo the results obtained the concentration 40 mg í1 forthe cuban varieties was selected The lncasoy-24 was the exception since it responded betterwith a concentration of 30 mg r1. These results favorthe development of new programs for genetic breedig of soybeans in Cuba. Keywords: somatic embryogenesis, Glycine max, plants regeneration
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