Effects of brassinosteroid on the induction of physiological changes in Helianthus annuus L. under copper stress
Angelika Filová | Oksana Sytar | Eleonóra Krivosudská
The purpose of our experiments was to consider sensitivity of the chosen 6 sunflower cultivars (Helianthus annuus L. cv. Belinda, cv. Codiwer, cv. ESPrim, cv. MAS 95, cv. MAS 97 and cv. Spirov) to copper ions with the possibility of elimination by means of brassinosteroids (BRs) on the ground of physiological characteristics (content of dry weight, amount of chlorophylls, proline content, Relative Water Content – RWC as well as malondialdehyde - MDA) and to show possible resistance mechanisms of this plant to copper ions. Lipid peroxidation (LP) is a biochemical marker for the free radical mediated injury. Character of changes in lipid peroxidation depends on intensity of influence stressor and from plant sensitivity. In leaves of experimental plants cultivars MAS 97 and SPIROV after Cu treatment by concentration 5 mM the level of malondialdehyde (MDA) content has been increased on 11% and 30% respectively. The higher MDA content has been observed in leaves of cultivar ESPrim. In the other experimental variants under Cu treatment content of MDA was on control level which evidence about more less sensitivity these cultivars to Cu treatment. The combination of Cu+ BRs has been shown MDA content in leaves of all experimental plants on control level which can evidence about protection effect of BRs under Cu treatment on the leaves of experimental cultivars of sunflower plants.
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