Regeneration of two commercial sugarcane cultivars (CP48-103 and CP69-1062) from terminal leaves derived explants
Sadeq Kalantar Hormozi | Mohammad Reza Siyah poush | Hamid Rajabi Memari | Hassan Hamdi | Mahmoud Sahmili | Jamil Hamoudi
Sugarcane is one of the most important industrial plants. There are many ways to improve the agronomic traits of this plant, such as genetic engineering and gene transfer methods. These modern techniques depend on optimizing the regeneration of plants from callus tissue. In this study, we attempted to optimize all different stages of tissue culture for regenerating the plants from callus tissue derived from terminal roll lives of two commercial sugarcane cultivars, CP48-10 and CP69-1062. Two types of cutting were applied, ring cutting and half-cylinder cutting from roll leaves. Among these cutting types, ring cutting from the roll leaves gives better results. Using solid MS medium with 3 mg per liter 2,4-D could attain the maximum callus production. To produce shoots from callus, the hormone combination of 1 mg per liter BAP and 2 mg per liter Kinetin showed the best response. Seedlings produced in MS medium containing 5 mg per liter IBA, 1 mg per liter Kinetin and 3 mg per liter NAA accompanied by 3 g per liter activated charcoal rendered the most root production. In the treatments with activated charcoal, the regenerated seedlings had superiority for the traits, days to root emergence, root length and number of roots in plants.
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