ANTIFUNGAL POTENTIAL OF ALCOHOLIC PLANT EXTRACTS IN REDUCING FUNGAL CONTAMINATION IN PHOENIX DACTYLIFERA TISSUE CULTURE
N. H. Hanoush
This study was conducted to investigation was carried out to evaluate the efficacy of certain plant extracts derived from pomegranate peels and black seeds in combating fungal issues in tissue culture, exploring their potential as natural substitutes for chemical treatments.The study was conducted in the fungi lab, University of Anbar, 2022-2023. In this study, three types of fungi were isolated from the plant tissues. Alcoholic extracts of pomegranate peels and black seed were used at different concentrations 0, 5, 10, 15 and 20% to inhibiting the growth of fungi isolated from tissue culture in PDA medium.The study results showed the isolation of three types of fungi associated with culture of plant tissues in vitro (Alternaria alternata, Asperigellus niger and Fusarium solani). The results showed the highest rate of growth inhibition at a 20% concentration, the alcoholic extracts of pomegranate peel and black seeds of isolates of fungi A. alternata, A. niger and F. solani were (73.89 and 73.63)%, (74.99 and 74.78)%, (77.42 and 78.00)%, respectively. The extracts from black seed and pomegranate peels exhibit the most effective fungistatic properties. These plants possess medicinal qualities, including antioxidant, antimicrobial, and anti-inflammatory attributes, largely attributed to their polyphenolic compounds. So that, can used these extracts to control on pathogen fungi to plant.
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