Hematology and Histopathology of Broiler Chickens Supplemented with Tuna Black Flour
OFP Rebolledo | JEM Barrera | JG Márquez | AR Vázquez | ACG Casillas
ABSTRACT The present study evaluated the effect of the sex and dietary intake of tuna black flour (TBF)on the hematology and histopathology of broiler chickens. A total of 200 sexed broilers (Ross 380) were distributed according to a completely randomized design into four treatments (25 males and 25 females/experimental group).Different levels of inclusion of TBF (0, 1, 2, and 3%) were offered as a source of ω-3 polyunsaturated fatty acids (ω-3 PUFAs), for 42 d. At the end of the experiment, blood samples and tissues were collected. The hematocrit value (Hemat), total protein (PROT-T), hemoglobin concentration (Hemog), erythrocytes concentration (Erythro) and total count and differential of leucocytes (Leuco): lymphocytes, heterophils, monocytes, eosinophils, and basophils were determined. Statistical analysis of the data was performed using the Analysis of Variance test, Duncan’s multiple range test, and descriptive analyses (p<0.05). Samples of heart, liver, and bursa of Fabricius were processed with the routine histological technique. The sex had no significant effect on the hematology. The feeding additional with 3% TBF had lower level of Hemat value and PROT-T. The Leuco had lower value with 1 and 2% of TBF. The Monocytes showed low value with 1 and 2% of TBF, and the eosinophils had high level with 1% of TBF. No cardiac lesions were detected in the broiler chickens in all treatments, normal deposition of fat in the epicardium, endocardium and pericardium were seen in all treatments.
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