Study of SNP 775C>T polymorphism within the bovine ITGB2 gene in Polish Black-and-White cattle and in local breeds of cattle
U. Czarnik | M. Galiński | T. Zabolewicz | Ch.S. Pareek
The present study addresses the characteristics of the frequency and segregation of alleles determining the SNP 775C>T polymorphism within the bovine ITGB2 gene in the Black-and-White cattle population as well as in two endemic breeds of Polish Red and Polish White-Back cattle population qualified to the international programme of genetic resource diversity preservation in farm animals. The SNP 775C>T polymorphism revealed three amplified restriction fragments of 31 bp, 77 bp and 108 bp, forming three genotypes CC (31 bp, 77 bp), CT (31 bp, 77 bp, 108 bp) and TT (108 bp). A group of randomly selected Black-and-White cows was characterized by a negligible percentage of homozygous genotypes TT (5.1%) and by a prevailing percentage of heterozygous CT (60.1%) and homozygous CC (34.8%). In contrast, the between breeds analysis revealed that cows involved in the programme of genetic diversity preservation had a high prevalence of homozygotes CC (Polish Red - 55.9%, Polish White-Back - 60.0%), and a low percentage of homozygotes TT (Polish Red - 8.8%, Polish White-Back - 5.7%) in comparison with the Black-and-White cattle population. The degree of homozygosity in groups of Polish White-Back cows (65.7%) and Polish Red cows (64.7%) was remarkably higher than that of the Black-and-White cows (39.9%).
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