Translation Elongation Factor 1-Alpha Sequencing Provides Reliable Tool for Identification of <i>Fusarium graminearum</i> Species Complex Members
Emre Yörük | Tapani Yli-Mattila
The <i>Fusarium graminearum</i> species complex (FGSC) is a worldwide phytopathogenic fungus of small grain cereals. Genetics and bioinformatics tools have been providing an efficient strategy for identifying FGSC. However, the potential reliability of <i>tef1−α</i> sequencing in FGSC members has not been well investigated. In this study, the <i>tef1−α</i> sequencing data of 246 FGSC members, one <i>F. culmorum</i>, and one <i>F. solani</i> isolate were subjected to distance-, character-, and PCA-based phylogenetic analysis. Linux terminals and the R programming language were used in phylogenetic analysis. The Unweighted Pair Group Method with Arithmetic Mean (UPGMA) and maximum likelihood methods produced relatively more homogenous <i>F. graminearum sensu stricto</i> (<i>Fgss</i>) and <i>F. asiaticum</i> isolates. <i>Fgss</i> and <i>F. asiaticum</i> isolates co-clustered in two separate sub-divisions in the ML and UPGMA methods, with significant differences in the Chi2 test (<i>p</i> < 0.05). PCA profiling revealed a low level of variation in FGSC members, with 99–99.5% percentages in axis 1. An increased number of taxa and isolates would be tested for <i>tef1−α</i> in future studies. To our knowledge, this is also the first study to combine phylogenetic methods with PCA tests for comprehensive characterization of FGSC members.
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