Determination of Artemisinin Content in Artemisia annua L .
Jambalsuren Bayarmaa | Gregorio De Zorzi
Artemisinin from dry leaves of Artemisia annua L. was extracted by supercritical fl uid extraction using CO 2 as the solvent and Microwave-assisted extraction by diff erent solvents as ethanol, chloroform, n-hexane and toluene. Extracts were analyzed in GC-MS system gas chromatograph, by comparison of the retention time with that of the standard artemisinin. Determination of arteannuin B, artemisinic acids were conducted by comparison of the spectrum with that of a library. The content of artemisinin was determined as 0.29-0.85% of dry weight for microwave-assisted extraction, and 0.32% for supercritical fl uid extraction, for arteannuin B as 0.18-1.23% and 1.18%, for artemisinic acid as 0.32-2.33% and 0.66%, respectively. The yield of total extract obtained by supercritical fl uid extraction was 1.70%, by microwave-assisted extraction - 1.33-8.62%. Best solvents for the artemisinin extraction were toluene, chloroform and ethanol.
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