Derivation of the clinical grade human embryonic stem cell line RCe016-A (RC-12)
P.A. De Sousa | B.J. Tye | K. Bruce | P. Dand | G. Russell | D.M. Collins | A. Greenshields | K. McDonald | H. Bradburn | A. Laurie | J.M. Downie | M. Bateman | A. Courtney
The human embryonic stem cell line RCe016-A (RC-12) was derived under quality assured compliance with UK regulations, EU Directives and International guidance for tissue procurement, processing and storage according to good manufacturing practice (GMP) standards. The cell line was derived from a cryopreserved blastocyst stage embryo voluntarily donated as surplus to fertility requirements following informed consent. RCe016-A (RC-12) shows normal pluripotency marker expression and differentiation to three germ layers in vitro. Karyology revealed a mixed male karyotype at early passage (P15), which resolved as normal 46XY by passage 33. Microsatellite PCR identity, HLA and blood group typing data is available.
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