Expression characteristics of OsRPL36A in rice
2024
CUI Zhuocheng | LI Yuechen | FAN Jinlan | ZENG Yuanhuan | LI Keke | TANG Huiwu
[ Objective ] As an important regulator involved in protein synthesis and gene expression , riboso- mal proteins are crucial for plant growth and development and stress response. In this study , a ribosomal protein family gene OsRPL36A was cloned from rice , and a preliminary study was performed for its bio- logical function , providing a basis for the study of OsRPL36A function. [ Methods ] Bioinformatics tech- niques were used to analysis gene structure , cis -regulatory elements , and evolution process of OsRPL36A . Meanwhile , the expression of OsRPL36A in different tissues and under diurnal rhythm , different hor- mones , and abiotic stresses were analyzed by quantitative real-time PCR ( qRT-PCR ) . [ Results ] ( 1 ) OsR- PL36A encoded 98 amino acids with a 297 bp coding region , which belonged to the ribosomal protein L36 supergene family , and was named as OsRPL36A . ( 2 ) The promoter region of OsRPL36A contained 3 rhythmic expression-related elements , 10 light-responsive elements , 14 hormone-responsive elements , and 27 environmental stress response elements. ( 3 ) The expression of OsRPL36A in leaves was relatively higher than that in other tissues and presented a typical rhythmic expression pattern. The expression of OsRPL36A was in- duced by IAA , high temperature , low temperature , osmotic stress , and so on. [ Conclusion ] OsRPL36A was highly expressed in leaves and presented a typical rhythmic expression pattern. OsRPL36A responded to IAA and might be involved in response to heat shock , low temperature , salt , and osmotic stresses.
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