Spinochrome Identification and Quantification in Pacific Sea Urchin Shells, Coelomic Fluid and Eggs Using HPLC-DAD-MS
Elena A. Vasileva | Natalia P. Mishchenko | Van T. T. Tran | Hieu M. N. Vo | Sergey A. Fedoreyev
The high-performance liquid chromatography method coupled with diode array and mass spectrometric detector (HPLC-DAD-MS) method for quinonoid pigment identification and quantification in sea urchin samples was developed and validated. The composition and quantitative ratio of the quinonoid pigments of the shells of 16 species of sea urchins, collected in the temperate (Sea of Japan) and tropical (South-China Sea) climatic zones of the Pacific Ocean over several years, were studied. The compositions of the quinonoid pigments of sea urchins <i>Maretia planulata</i>, <i>Scaphechinus griseus</i>, <i>Laganum decagonale</i> and <i>Phyllacanthus imperialis</i> were studied for the first time. A study of the composition of the quinonoid pigments of the coelomic fluid of ten species of sea urchins was conducted. The composition of quinonoid pigments of <i>Echinarachnius parma</i> jelly-like egg membrane, of <i>Scaphechinus mirabilis</i> developing embryos and pluteus, was reported for the first time. In the case of <i>Scaphechinus mirabilis</i>, we have shown that the compositions of pigment granules of the shell epidermis, coelomic fluid, egg membrane, developing embryos and pluteus are different, which should enable a fuller understanding of the functions of pigments at different stages of life.
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