Biosynthesis of Fatty Acid Derivatives by Recombinant <i>Yarrowia lipolytica</i> Containing MsexD2 and MsexD3 Desaturase Genes from <i>Manduca sexta</i>
Jaroslav Hambalko | Peter Gajdoš | Jean-Marc Nicaud | Rodrigo Ledesma-Amaro | Michal Tupec | Iva Pichová | Milan Čertík
One of the most interesting groups of fatty acid derivates is the group of conjugated fatty acids from which the most researched include: conjugated linoleic acid (CLA) and conjugated linolenic acid (CLNA), which are associated with countless health benefits. Sex pheromone mixtures of some insect species, including tobacco horn-worm (<i>Manduca sexta</i>), are typical for the production of uncommon C16 long conjugated fatty acids with two and three conjugated double bonds, as opposed to C18 long CLA and CLNA. In this study, <i>M. sexta</i> desaturases MsexD2 and MsexD3 were expressed in multiple strains of <i>Y. lipolytica</i> with different genotypes. Experiments with the supplementation of fatty acid methyl esters into the medium resulted in the production of novel fatty acids. Using GCxGC-MS, 20 new fatty acids with two or three double bonds were identified. Fatty acids with conjugated or isolated double bonds, or a combination of both, were produced in trace amounts. The results of this study prove that <i>Y. lipolytica</i> is capable of synthesizing C16-conjugated fatty acids. Further genetic optimization of the <i>Y. lipolytica</i> genome and optimization of the fermentation process could lead to increased production of novel fatty acid derivatives with biotechnologically interesting properties.
Показать больше [+] Меньше [-]Библиографическая информация
Эту запись предоставил Directory of Open Access Journals