Structural Characterization and In Vivo Anti-Inflammatory Activity of Fucoidan from <i>Cystoseira crinita</i> (Desf.) Borry
Elisaveta Apostolova | Paolina Lukova | Alexandra Baldzhieva | Cédric Delattre | Roland Molinié | Emmanuel Petit | Redouan Elboutachfaiti | Mariana Nikolova | Ilia Iliev | Marianna Murdjeva | Vesela Kokova
The aim of this study was to evaluate the effects of fucoidan isolated from <i>C. crinita</i> on histamine-induced paw inflammation in rats, and on the serum levels of TNF-α, IL-1β, IL-6, and IL-10 in rats during systemic inflammation response. The levels of TNF-α in a model of acute peritonitis in rats were also investigated. The isolated crude fucoidan was identified as a sulfated xylogalactofucan with high, medium, and low molecular weight fractions and a content of fucose of 39.74%, xylose of 20.75%, and galactose of 15.51%. Fucoidan from <i>C. crinita</i> showed better anti-inflammatory effects in the rat paw edema model, and this effect was present during all stages of the experiment. When compared to controls, a commercial fucoidan from <i>F. vesiculosus,</i> the results also displayed anti-inflammatory activity on the 60th, 90th, and 120th minute of the experiment. A significant decrease in serum levels of IL-1β in rats treated with both doses of <i>C. crinita</i> fucoidan was observed in comparison to controls, whereas TNF-α concentrations were reduced only in the group treated with fucoidan from <i>C. crinita</i> at the dose of 25 mg/kg bw. In the model of carrageenan-induced peritonitis, we observed a tendency of decrease in the levels of the pro-inflammatory cytokine TNF-α in peritoneal fluid after a single dose of <i>C. crinita</i> fucoidan, but this did not reach the statistical significance margin. Single doses of <i>C. crinita</i> fucoidan did not alter serum levels of the anti-inflammatory cytokine IL-10 in animals with lipopolysaccharide-induced systemic inflammation.
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