<i>In</i> <i>Vitro</i> Anthelmintic Activity of Sea Buckthorn (<i>Hippophae rhamnoides</i>) Berry Juice against Gastrointestinal Nematodes of Small Ruminants
Michela Maestrini | Cristina Forzato | Simone Mancini | Ylenia Pieracci | Stefania Perrucci
Gastrointestinal nematodes are one of the major threats in small ruminant breeding. Their control is difficult due to the development of anthelmintic resistance, and the search for new molecules endowed with anthelmintic activity (AH) is considered a priority. In this context, we evaluated the <i>in vitro</i> AH activity of two commercial sea buckthorn (<i>Hippophae rhamnoides</i>) berry juices, namely SBT and SBF. The <i>in vitro</i> evaluation was based on the egg-hatch test and larval exsheathment assay at different concentrations. Data were statistically analysed, and the EC<sub>50</sub> was calculated. Chemical analyses were performed to evaluate the total polyphenol content of the juices and chemical profile of the most represented compounds. The role of the polyphenolic fraction in the anthelmintic activity of the juices was also assessed. At the highest concentrations, the activity of SBT was high in both tests and comparable to that observed in the thiabendazole-treated positive controls, while SBF showed a lower efficacy. Glycosylated isorhamnetin and quercetin were the most represented polyphenolic compounds in both juices. In conclusion, both <i>H. rhamnoides</i> berry juices tested in this study showed interesting anthelmintic properties <i>in vitro</i>.
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