Lipid and Fatty Acids Accumulation Features of <em>Entomoneis</em> cf. <em>paludosa</em> during Exponential and Stationary Growth Phases in Laboratory Culture
Yekaterina Bedoshvili | Yulia Podunay | Alyona Nikonova | Artyom Marchenkov | Elvira Bairamova | Nikolai Davidovich | Yelena Likhoshway
Diatoms are capable of accumulating substantial amounts of triacylglycerides in their cells, which differ in the composition of fatty acids depending on the conditions of cultivation, making them attractive subjects in biotechnology. In the present study, we characterized the structural features of lipid bodies in the diatom <i>Entomoneis</i> cf. <i>paludosa</i> (W. Smith) Reimer strain 8.0727-B and revealed the peculiarities of fatty acid composition in cultures during the stationary and exponential growth phases. Laser scanning confocal microscopy revealed an increased number of lipid bodies in the cytoplasm during the stationary phase of culture growth. Electron microscopy of ultrathin sections showed that an extreme increase in the number and size of plastoglobules in the cells occurs in the stationary phase of culture growth. The gas chromatography with mass spectrometric detection method revealed differences in the fatty acid composition depending on the growth phase. The studied strain can be recommended as a source of hexadecanoic and octadecanoic fatty acids from the culture during the stationary growth phase, as well as eicosapentaenoic fatty acid from the culture during the exponential growth phase.
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