Studies on the Genus <i>Pyrenopolyporus</i> (Hypoxylaceae) in Thailand Using a Polyphasic Taxonomic Approach
Sarunyou Wongkanoun | Boonchuai Chainuwong | Noppol Kobmoo | Sittiruk Roytrakul | Sayanh Somrithipol | Jennifer Luangsa-ard | Esteban Charria-Girón | Prasert Srikitikulchai | Marc Stadler
Over the past two decades, hypoxylaceous specimens were collected from several sites in Thailand. In this study, we examined their affinity to the genus <i>Pyrenopolyporus</i> using macroscopic and microscopic morphological characters, dereplication of their stromatal secondary metabolites using ultrahigh performance liquid chromatography coupled to diode array detection and ion mobility tandem mass spectrometry (UHPLC-DAD-IM-MS/MS), and molecular phylogenetic analyses. We describe and illustrate five novel species and a new record for the country, present multi-locus phylogenetic analyses that show the distinction between the proposed species, and provide proteomic profiles of the fungi using matrix associated laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF/MS) for the first time. Based on our findings, this strategy is useful as a complementary tool to distinguish species between <i>Daldinia</i> and <i>Pyrenopolyporus</i> in a consistent way with the phylogenetic analysis.
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