Selection of Autochthonous Yeasts Isolated from the Intestinal Tracts of Cobia Fish (<i>Rachycentron canadum)</i> with Probiotic Potential
Samira Reinoso | María Soledad Gutiérrez | Cristóbal Domínguez-Borbor | Wilfrido Argüello-Guevara | Milton Bohórquez-Cruz | Stanislaus Sonnenholzner | Daniela Nova-Baza | Claudia Mardones | Paola Navarrete
Some yeast strains have been proposed as probiotics to improve the health of cultured fish. Cobia is a tropical benthopelagic fish species with potential for marine aquaculture; however, one of the main limitations to its large-scale production is the high mortality of fish larvae. In this study, we evaluated the probiotic potential of autochthonous yeasts from the intestines of cobia. Thirty-nine yeast isolates were recovered from the intestinal mucosa of 37 adult healthy cobia by culture methods. Yeasts were identified by sequencing of the ITS and D1/D2 regions of the 28S rRNA gene and typed by RAPD-PCR using the M13 primer. Yeast strains with unique RAPD patterns were characterized in terms of their cell biomass production ability; anti-<i>Vibrio</i>, enzymatic, and hemolytic activity; biofilm production; hydrophobicity; autoaggregation; polyamine production; safety; and protection of cobia larvae against saline stress. <i>Candida haemuloni</i> C27 and <i>Debaryomyces hansenii</i> C10 and C28 were selected as potential probiotics. They did not affect the survival of larvae and showed biomass production >1 g L<sup>−1</sup>, hydrophobicity >41.47%, hemolytic activity γ, and activity in more than 8 hydrolytic enzymes. The results suggest that the selected yeast strains could be considered as potential probiotic candidates and should be evaluated in cobia larvae.
Показать больше [+] Меньше [-]Ключевые слова АГРОВОК
Библиографическая информация
Эту запись предоставил Directory of Open Access Journals