To evaluate the effect of iron nanoparticles on expression of TEM and SHV genes in Klebsiella pneumoniae isolated from clinical samples using Real Time PCR
2019
najmeh tahmasebi | babak kheirkhah
Background: Klebsiella pneumoniae is a germ-negative pathogen and a common cause of nosocomial infections. Increasing the emergence of multi-drug resistance in Klebsiella pneumonia has limited therapeutic options for this bacterium. The purpose of this study was to evaluate the effect of iron nanoparticles on expression of TEM and SHV genes in Klebsiella pneumoniae isolated from clinical samples using Real- Time PCR. Materials and Methods: A total of 50 strains of Klebsiella pneumoniae were collected from patients admitted to the intensive care unit of hospitals in Kerman on 6 months. The isolates were identified as K. pneumoniae, based on the biochemical tests embedded in the API-20E system. Antibiotic susceptibility test was performed by disc diffusion method. For molecular identification of TEM and SHV genes, PCR was performed. To determine the effect of iron nanoparticles on the strains, a dilution broth method was used according to CLSI standard and finally, Real-Time PCR was performed. Results: In this study, 46 isolates were positive for ESBL producing phenotypes. In the 46 isolates of ESBL producing, 48% (22 cases) had the SHV gene , 13% (6 cases) had the TEM gene and 39% (18 cases) had both SHV + TEM genes. Iron nanoparticles could degrade the expression of TEM and SHV genes in the clinical medium in Klebsiella pneumoniae. Conclusion: Due to the effectiveness of iron nanoparticles on positive beta-lactamases Klebsiellas, can use iron nanoparticles as a medicine dose.
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