Generation of a TLE3 heterozygous knockout human embryonic stem cell line using CRISPR-Cas9
2016
Anne M. Bara | Angelica Messana | Amanda Herring | Dane Z. Hazelbaker | Kevin Eggan | Lindy E. Barrett
Here, we generated a monoallelic mutation in the TLE3 (Transducin Like Enhancer of Split 3) gene using CRISPR-Cas9 editing in the human embryonic stem cell (hESC) line WA01. The heterozygous knockout cell line, TLE3-447-D08-A01, displays partial loss of TLE3 protein expression while maintaining pluripotency, differentiation potential and genomic integrity.
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