Chemical process to improve natural grapevine-cane extract effectivity against powdery mildew and grey mould
Tristan Richard | Toni El Khawand | David Taillis | Grégory Da Costa | Eric Pedrot | Stéphanie Cluzet | Alain Decendit | Josep Valls Fonayet
Grapevine canes are vine growing byproducts studied for their antimicrobial activities. These properties are directly connected to the stilbene content; oligomeric stilbenes being the most active. In this study, we propose a chemical process, based on oxidative coupling, using metals to increase the oligostilbene rate and the biological effectivity of cane extract against grapevine pathogens. A total of ten compounds were obtained and identified by combining LCMS and NMR spectroscopies, including four newly reported compounds: trans-oxistilbenin C, trans-oxistilbenin D, and cis- and trans-oxistilbenin E. The extract and the main stilbene formed were evaluated for their preventive effects on Plasmopara viticola and Botrytis cinerea growth. The processed extract was highly effective against both pathogens.
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