CRISPR/Cas9-Mediated Mutagenesis of <i>RCO</i> in <i>Cardamine hirsuta</i>
Claire Lessa Alvim Kamei | Bjorn Pieper | Stefan Laurent | Miltos Tsiantis | Peter Huijser
The small crucifer <i>Cardamine hirsuta</i> bears complex leaves divided into leaflets. This is in contrast to its relative, the reference plant <i>Arabidopsis thaliana</i>, which has simple leaves. Comparative studies between these species provide attractive opportunities to study the diversification of form. Here, we report on the implementation of the CRISPR/Cas9 genome editing methodology in <i>C. hirsuta</i> and with it the generation of novel alleles in the <i>RCO</i> gene, which was previously shown to play a major role in the diversification of form between the two species. Thus, genome editing can now be deployed in <i>C. hirsuta</i>, thereby increasing its versatility as a model system to study gene function and evolution.
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