Biotic and Abiotic Elicitors of Stilbenes Production in <i>Vitis vinifera</i> L. Cell Culture
Martin Sák | Ivana Dokupilová | Šarlota Kaňuková | Michaela Mrkvová | Daniel Mihálik | Pavol Hauptvogel | Ján Kraic
The in vitro cell cultures derived from the grapevine (<i>Vitis vinifera</i> L.) have been used for the production of stilbenes treated with different biotic and abiotic elicitors. The red-grape cultivar Váh has been elicited by natural cellulose from <i>Trichoderma viride</i>, the cell wall homogenate from <i>Fusarium oxysporum</i> and synthetic jasmonates. The sodium-orthovanadate, known as an inhibitor of hypersensitive necrotic response in treated plant cells able to enhance production and release of secondary metabolite into the cultivation medium, was used as an abiotic elicitor. Growth of cells and the content of phenolic compounds <i>trans</i>-resveratrol, <i>trans</i>-piceid, δ-viniferin, and ɛ-viniferin, were analyzed in grapevine cells treated by individual elicitors. The highest accumulation of analyzed individual stilbenes, except of trans-piceid has been observed after treatment with the cell wall homogenate from <i>F. oxysporum</i>. Maximum production of trans-resveratrol, δ- and ɛ-viniferins was triggered by treatment with cellulase from <i>T. viride</i>. The accumulation of trans-piceid in cell cultures elicited by this cellulase revealed exactly the opposite effect, with almost three times higher production of trans-resveratrol than that of <i>trans</i>-piceid. This study suggested that both used fungal elicitors can enhance production more effectively than commonly used jasmonates.
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