BnGF14-2c Positively Regulates Flowering via the Vernalization Pathway in Semi-Winter Rapeseed
Shihang Fan | Hongfang Liu | Jing Liu | Wei Hua | Jun Li
14-3-3s are general regulatory factors (GF14s or GRFs) involved in a variety of physiological regulations in plants, including the control of flowering time. However, there are poorly relevant reports in rapeseed so far. In this study, we identified a homologous <i>14-3-3</i> gene <i>BnGF14-2c</i> (<i>AtGRF2</i>_Like in <i>Brassica napus</i>) in rapeseed based on bioinformatic analysis by using the sequences of the flowering-related <i>14-3-3s</i> in other plant species. Then, we found that overexpression of <i>BnGF14-2c</i> in the semi-winter rapeseed “93275” promoted flowering without vernalization. Moreover, both yeast two-hybrid and bimolecular fluorescence complementation analysis indicated that BnGF14-2c may interact with two vernalization-related flowering regulators BnFT.A02 and BnFLC.A10., respectively. qPCR analysis showed that the expression of <i>BnFT</i> (<i>AtFT</i>_Like) was increased and the expression of two selected vernalization-related genes were reduced in the overexpression transgenic plants. Further investigation on subcellular localization demonstrated that BnGF14-2c localized in the nucleus and cytoplasm. The results of RNA-seq analysis and GUS staining indicated that <i>BnGF14-2c</i> is ubiquitously expressed except for mature seed coat. In general, the interaction of 14-3-3 and FLC was firstly documented in this study, indicating BnGF14-2c may act as a positive regulator of flowering in rapeseed, which is worthy for more in-depth exploration.
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