Optimization of the Extraction Procedure for the Phenolic-Rich <i>Glechoma hederacea</i> L. Herb and Evaluation of Its Cytotoxic and Antioxidant Potential
Karolina Grabowska | Kinga Amanowicz | Paweł Paśko | Irma Podolak | Agnieszka Galanty
The dried <i>Glechoma hederacea</i> L. herb has a long history of use in traditional medicine. Its therapeutic potential is related to the presence of phenolic compounds. To optimize extraction efficiency the effect of the use of different techniques (HRE—heat reflux extraction, I/ME—infusion combined with maceration, UE—sonication and SE—Soxhlet extraction), various solvents (water and ethanol) and processing time (15 min to 2 h) on phenolics content was investigated. The HPLC method was applied to determine and compare the content of phenolic acids (rosmarinic, chlorogenic, protocatechuic) and flavonoids (rutin, isoquercetin) in the extracts. Furthermore, the cytotoxic activity of the extracts was examined for the first time against human cancer and normal cells of skin origin (A375, HTB140, HaCaT) and gastrointestinal origin (Caco-2 and HT-29, HepG2). In addition, the antioxidant potential was evaluated using the DPPH and FRAP method. The I/ME-water and HRE/ethanol procedures turned out to be optimal for obtaining extracts of dried <i>G. hederacea</i> L. herb rich in bioactive phenolics. These extracts exhibited high antioxidant activity, correlated with the content of the compounds analyzed. Furthermore, the extracts of the dried <i>Glechoma</i> herb were not toxic to normal human cells, indicating its safe use both internally and externally.
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