The Influence of Seasonality on Secondary Metabolite Profiles and Neuroprotective Activities of Moss <i>Hypnum cupressiforme</i> Extracts: In Vitro and In Silico Study
Tanja M. Lunić | Marija R. Mandić | Mariana M. Oalđe Pavlović | Aneta D. Sabovljević | Marko S. Sabovljević | Biljana Đ. Božić Nedeljković | Bojan Đ. Božić
Numerous representatives of mosses, including <i>Hypnum cupressiforme</i>, have been used to alleviate different inflammation-related conditions. However, the mode of action underlying this anti-inflammatory potential has been poorly understood. Moreover, the influence of seasonality on the chemical composition and biological activity of mosses is generally overlooked. This study aimed to investigate the influence of seasonal changes (spring, summer, and autumn) on secondary metabolite composition and biological activities of ethyl acetate <i>H. cupressiforme</i> extracts. Antioxidant activity was measured using β-carotene bleaching assay, while MTT, NBT, ELISA, and Griess assays were carried out to explore the anti-neuroinflammatory and neuroprotective potential of extracts. Inhibitory activities on acetylcholinesterase and tyrosinase were assessed experimentally and by docking analysis. The highest content of secondary metabolites and antioxidant activity were observed in moss during the summer. Extracts inhibited the secretion of ROS, NO, TNF-α, and IL-6, alleviating the inflammatory potential of H<sub>2</sub>O<sub>2</sub> and LPS in microglial and neuronal cells. Strong inhibitory effects on acetylcholinesterase and tyrosinase were observed in vitro. Docking analyses revealed high-affinity interactions of secondary metabolites present in <i>H. cupressiforme</i> with important enzyme residues. Altogether, these results reveal the neuroprotective potential and the significance of seasonal fluctuations on secondary metabolite content and biological activities in moss <i>H. cupressiforme</i>.
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