In Vitro Regeneration of <i>Miscanthus</i> x <i>giganteus</i> through Indirect Organogenesis: Effect of Explant Type and Growth Regulators
Aušra Blinstrubienė | Inga Jančauskienė | Natalija Burbulis
<i>Miscanthus</i> x <i>giganteus</i> is a spontaneous sterile hybrid therefore the creation of useful genetic diversity by conventional breeding methods is restricted. Plant regeneration through indirect organogenesis may be a useful approach to create genetic variability of this important agricultural crop. The present study aimed to evaluate the effect of the explant type and growth regulators on indirect organogenesis of <i>Miscanthus</i> x <i>giganteus</i> and to determine the ploidy level of plant regenerants by flow cytometry. On average, the highest percentage of morphogenic callus tested explants formed in the medium supplemented with 2.5 mg L<sup>–1</sup> IBA + 0.1 mg L<sup>–1</sup> BAP + 4.0 mg L<sup>–1</sup> <span style="font-variant: small-caps;">l</span>-proline. The most intensive secondary differentiation of callus cells was observed in the medium supplemented with 4.0 mg L<sup>–1</sup> ZEA + 1.0 mg L<sup>–1</sup> NAA. The highest root formation frequency with the highest number of roots was determined in the MS nutrient medium supplemented with 0.4 mg L<sup>–1</sup> IBA, where more than 95% of plant regenerants survived and were growing normally.
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