<i>CAD</i> Genes: Genome-Wide Identification, Evolution, and Their Contribution to Lignin Biosynthesis in Pear (<i>Pyrus bretschneideri</i>)
Kaijie Qi | Xiaofei Song | Yazhou Yuan | Jianping Bao | Xin Gong | Xiaosan Huang | Shahrokh Khanizadeh | Shaoling Zhang | Shutian Tao
The synthetic enzyme cinnamyl alcohol dehydrogenase (CAD) is involved in responses to various stresses during plant growth. It regulates the monolignol biosynthesis and catalyzes hydroxyl cinnamaldehyde reduction to the corresponding alcohols. Although the CAD gene families have been explored in some species, little known is in Rosaceae. In this study, we identified 149 genes in <i>Pyrus bretschneideri</i> (<i>PbrCAD</i>), <i>Malus domestica</i> (<i>MDPCAD</i>), <i>Prunus mume</i> (<i>PmCAD</i>) and <i>Fragaria vesca</i> (<i>mrnaCAD</i>). They were phylogenetically clustered into six subgroups. All CAD genes contained ADH-N and ADH-zinc-N domains and were distributed on chromosomes unevenly. Dispersed and WGD/segmental duplications accounted the highest number of evolutionary events. Eight collinear gene pairs were identified among the four Rosaceae species, and the highest number was recorded in pear as five pairs. The five <i>PbrCAD</i> gene pairs had undergone purifying selection under Ka/Ks analysis. Furthermore, nine genes were identified based on transcriptomic and stone cell content in pear fruit. In qRT-PCR, the expression patterns of <i>PbrCAD1</i>, <i>PbrCAD20</i>, <i>PbrCAD27</i>, and <i>PbrCAD31</i> were consistent with variation in stone cell content during pear fruit development. These results will provide valuable information for understanding the relationship between gene expressions and stone cell number in fruit.
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