Mitochondrial Genome of <i>Fagus sylvatica</i> L. as a Source for Taxonomic Marker Development in the Fagales
Malte Mader | Hilke Schroeder | Thomas Schott | Katrin Schöning-Stierand | Ana Paula Leite Montalvão | Heike Liesebach | Mirko Liesebach | Barbara Fussi | Birgit Kersten
European beech, <i>Fagus sylvatica</i> L., is one of the most important and widespread deciduous tree species in Central Europe and is widely managed for its hard wood. The complete DNA sequence of the mitochondrial genome of <i>Fagus sylvatica</i> L. was assembled and annotated based on Illumina MiSeq reads and validated using long reads from nanopore MinION sequencing. The genome assembled into a single DNA sequence of 504,715 bp in length containing 58 genes with predicted function, including 35 protein-coding, 20 tRNA and three rRNA genes. Additionally, 23 putative protein-coding genes were predicted supported by RNA-Seq data. Aiming at the development of taxon-specific mitochondrial genetic markers, the tool SNPtax was developed and applied to select genic SNPs potentially specific for different taxa within the Fagales. Further validation of a small SNP set resulted in the development of four CAPS markers specific for <i>Fagus</i>, Fagaceae, or Fagales, respectively, when considering over 100 individuals from a total of 69 species of deciduous trees and conifers from up to 15 families included in the marker validation. The CAPS marker set is suitable to identify the genus <i>Fagus</i> in DNA samples from tree tissues or wood products, including wood composite products.
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