Effects of <i>NtSPS1</i> Overexpression on Solanesol Content, Plant Growth, Photosynthesis, and Metabolome of <i>Nicotiana tabacum</i>
Ning Yan | Xiaolei Gai | Lin Xue | Yongmei Du | John Shi | Yanhua Liu
<i>Nicotiana tabacum</i> solanesyl diphosphate synthase 1 (NtSPS1) is the key enzyme in solanesol biosynthesis. However, changes in the solanesol content, plant growth, photosynthesis, and metabolome of tobacco plants after <i>NtSPS1</i> overexpression (OE) have not been previously reported. In the present study, these parameters, as well as photosynthetic gas exchange, chlorophyll content, and chlorophyll fluorescence parameters, were compared between <i>NtSPS1</i> OE and wild type (WT) lines of tobacco. As expected, <i>NtSPS1</i> OE significantly increased solanesol content in tobacco leaves. Although <i>NtSPS1</i> OE significantly increased leaf growth, photosynthesis, and chlorophyll content, the chlorophyll fluorescence parameters in the leaves of the <i>NtSPS1</i> OE lines were only slightly higher than those in the WT leaves. Furthermore, <i>NtSPS1</i> OE resulted in 64 differential metabolites, including 30 up-regulated and 34 down-regulated metabolites, between the OE and WT leaves. Pathway enrichment analysis of these differential metabolites identified differentially enriched pathways between the OE and WT leaves, e.g., carbon fixation in photosynthetic organisms. The maximum carboxylation rate of RuBisCO and the maximum rate of RuBP regeneration were also elevated in the <i>NtSPS1</i> OE line. To our knowledge, this is the first study to confirm the role of <i>NtSPS1</i> in solanesol biosynthesis and its possible functional mechanisms in tobacco.
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