Effect of Beta-Lactam Antibiotics on Microspore Embryogenesis in <i>Brassica</i> Species
Anna Mineykina | Daria Shumilina | Ludmila Bondareva | Alexey Soldatenko | Elena Domblides
Antibiotics are widely applied for plant cultivation in vitro to eliminate bacterial contamination. However, they can have both positive and negative effects on the cells of cultivated plants, and these effects largely depend on the type antibiotic used and its concentration. The objective of the present study was to estimate the effect of β-lactam antibiotics ampicillin (Amp) and cefotaxime (Cef) on microspore embryogenesis induction in vitro in the <i>Brassica</i> species. The performed experiments confirmed cefotaxime inhibits microspores in <i>B. napus</i> and <i>B. oleracea</i>, even in concentrations as low as 50 mg/L. The highest embryo yield was obtained for <i>B. napus</i> in the NLN-13 medium with added ampicillin in concentrations of 50–100 mg/L as an antimicrobial agent. This embryo yield was significantly higher than that obtained in a medium without supplemented antibiotics and two times higher than in the medium with added cefotaxime. Analogous results were obtained for <i>B. oleracea</i> and <i>B. rapa</i>.
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