<i>Xanthomonas euvesicatoria</i>-Specific Bacteriophage BsXeu269p/3 Reduces the Spread of Bacterial Spot Disease in Pepper Plants
Elena Shopova | Liliana Brankova | Sergei Ivanov | Zoltan Urshev | Lyudmila Dimitrova | Melani Dimitrova | Petya Hristova | Yoana Kizheva
The present study was focused on the pathosystem pepper plants (<i>Capsicum annuum</i> L.)-phytopathogenic bacterium <i>X. euvesicatoria</i> (wild strain 269p)-bacteriophage BsXeu269p/3 and the possibility of bacteriophage-mediated biocontrol of the disease. Two new model systems were designed for the monitoring of the effect of the phage treatment on the infectious process in vivo. The spread of the bacteriophage and the pathogen was monitored by qPCR. A new pair of primers for phage detection via qPCR was designed, as well as probes for TaqMan qPCR. The epiphytic bacterial population and the potential bacteriolytic effect of BsXeu269p/3 in vivo was observed by SEM. An aerosol-mediated transmission model system demonstrated that treatment with BsXeu269p/3 reduced the amount of <i>X. euvesicatoria</i> on the leaf surface five-fold. The needle-pricking model system showed a significant reduction of the amount of the pathogen in infectious lesions treated with BsXeu269p/3 (av. 59.7%), compared to the untreated control. We found that the phage titer is 10-fold higher in the infection lesions but it was still discoverable even in the absence of the specific host in the leaves. This is the first report of in vivo assessment of the biocontrol potential of locally isolated phages against BS pathogen <i>X. euvesicatoria</i> in Bulgaria.
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