Characterization of the Slovene Autochthonous Rose Hybrid <i>Rosa pendulina</i> × <i>spinosissima</i> (<i>Rosa reversa</i> Waldst. and Kit) Using Biochemical Patterns of the Plant Blossoms
Nina Kunc | Metka Hudina | Jože Bavcon | Branko Vreš | Zlata Luthar | Kristina Gostinčar | Maja Mikulič-Petkovšek | Gregor Osterc | Blanka Ravnjak
The <i>Rosa</i> genus is characterized by great variability and, consequently, they easily hybridize. The petals of <i>R. pendulina</i>, <i>R. spinosissima</i> and their hybrid <i>Rosa pendulina</i> × <i>spinosissima</i>, collected in western Slovenia, were included in the research. We performed morphometric analysis using keys to determine roses and genetic analysis to determine the genome size. The phenolic compound content in petals of all rose flowers was measured by liquid chromatography and mass spectrometry (HPLC-MS). Using flow cytometry, we could confirm the native hybridization process due to the amount of 2C DNA. The value of <i>R. pendulina</i> was 1.71 pg, of <i>R. spinosissima</i> 1.60 pg and of the hybrid 1.62 pg. The value for the hybrid was close to values measured for parent plants and, at the same time, those values of parent plants significantly differed from each other. Our results showed that the content of phenolic compounds in petals decreased after crossing. We found that the highest total phenolic content (178.9 g/kg FW) was measured in <i>R. spinossisima</i>, the lowest content was analyzed for the hybrid (84.36 g/kg FW) and the content for <i>R. pendulina</i> was between these two values (110.58 g/kg FW). The content of flavanols and flavonols was lowest in the hybrid petals, whereas the content of anthocyanins was highest in the hybrid petals.
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