Improving Fermentation of Steamed Stalk to Feed Using Candida utilis and Pachysolen tannophilus
Chen He | Mei Sha | Dai Chunji | He Yunxia | Yu Jianjun
In order to improve the protein content of straw feed and reduce the amount of nutrients added, in this paper, the cell wall structure of corn stalk was destroyed by thermophilic digestion and the polysaccharide was degraded into monosaccharide by complex enzyme, and then transformed into bacterial protein by double strain Candida utilis 1807 and Pachysolen tannophilus 1771 fermentation. Single factor experiments and orthogonal test were made to obtain the best process for the feed of double-bacteria synchronous fermentation of stalks. The optimum amount of each nutrient and the inoculation amounts of double bacteria (accounts for the percentage of the original dry straw quality): ammonium sulfate 6.79%, urea 2.72%, yeast powder 1.63%, magnesium sulfate 0.27%, Candida utilis 54.31% and Pachysolen tannophilus 54.31%; The operational parameters of fermentation process were: fermentation temperature 29°C, rotate speed 100 r/min and fermentation time 55 hours. The yield of stalk feed and crude protein was 82.04%, 23.33%, respectively. The crude protein content of stalk feed was 28.44%, which was 4.33 times of original dry stalk. The results showed that the multi-strain distribution and degradation of protein production provide important significance for corn straw bio-utilization.
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