Identification and Comparative Analysis of the Rosaceae RCI2 Gene Family and Characterization of the Cold Stress Response in <i>Prunus mume</i>
Lichen Yang | Ping Li | Like Qiu | Sagheer Ahmad | Jia Wang | Tangchun Zheng
Rare cold inducible 2 (RCI2) proteins are a group of low molecular weight proteins that widely exist in various tissues of plants and play crucial roles in plant growth and development and abiotic stress responses. Genome-wide identification and analysis of <i>RCI2</i> have not been documented in Rosaceae plants. Therefore, we identified 23 <i>RCI2</i> genes from seven Rosaceae plants, which were classified into three subfamilies. The RoRCI2 protein encodes a highly conserved domain of Pmp3. Three homologous <i>PmRCI2</i>s genes from <i>Prunus mume</i> were cloned and named <i>PmRCI2-1</i>, <i>PmRCI2-2</i>, and <i>PmRCI2-3</i>. The results of subcellular localization prediction showed that three PmRCI2s localized to membrane structures, and the abscisic acid response element were found to have the largest number in the promoter sequences of <i>PmRCI2</i>s. The results of quantitative real-time PCR (qRT-PCR) showed that <i>PmRCI2-3</i> was significantly induced by low temperature and highly expressed in stems and buds during the endodormancy stage. Our study improves the understanding of the RCI2 family of Rosaceae plants regarding the cold responses and provides a theoretical basis for the cold-resistant breeding of <i>P. mume</i>.
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