Shortening the Vegetative Growth Stage of <i>Phalaenopsis</i> Queen Beer ‘Mantefon’ by Controlling Light with Calcium Ammonium Nitrate Levels under Enriched CO<sub>2</sub>
Ah Ram Cho | Sun Woo Chung | Yoon Jin Kim
The vegetative growth, photosynthetic, and stomatal characteristics were investigated in <i>Phalaenopsis</i> Queen Beer ‘Mantefon’ to determine light’s influence with calcium ammonium nitrate (CAN) levels under 800 μmol·mol<sup>−1</sup> CO<sub>2</sub>. Two lights (150 ± 20 and 300 ± 20 μmol·m<sup>−2</sup>·s<sup>−1</sup>) and CAN levels were employed for 40 weeks: calcium, ammonium, and nitrate levels by 0.90, 0.55, and 2.97 mmol·L<sup>−1</sup> (CAN1), 8.63, 1.11, and 6.05 mmol·L<sup>−1</sup> (CAN2), 12.80, 1.72, and 9.13 mmol·L<sup>−1</sup> (CAN3), and 18.80, 2.27, and 12.20 mmol·L<sup>−1</sup> (CAN4), respectively. The number of leaves increased in the plants grown at 300 ± 20 μmol·m<sup>−2</sup>·s<sup>−1</sup> with CAN1 compared to control. Plants grown at 300 ± 20 μmol·m<sup>−2</sup>·s<sup>−1</sup> with CAN4 had the lowest number of leaves among all plants. The time to the mature leaf span decreased in the plants grown at 300 ± 20 μmol·m<sup>−2</sup>·s<sup>−1</sup> with CAN1. The net CO<sub>2</sub> uptake was higher in the plants grown at 300 ± 20 μmol·m<sup>−2</sup>·s<sup>−1</sup> than those grown at 150 ± 20 μmol·m<sup>−2</sup>·s<sup>−1</sup> with CAN1–3 conditions. The water-use efficiency is higher in the plants grown with CAN1 than those with CAN2–4 at 300 ± 20 μmol·m<sup>−2</sup>·s<sup>−1</sup>. The maximum stomatal aperture was the largest in the plants grown at 300 ± 20 μmol·m<sup>−2</sup>·s<sup>−1</sup> with CAN1–2 among all plants. Consequently, light levels of 300 ± 20 μmol·m<sup>−2</sup>·s<sup>−1</sup> in <i>Phalaenopsis</i> Queen Beer ‘Mantefon’ must be accompanied by nutrient CAN1 to improve photosynthesis and stomatal activity and promote leaf growth under 800 μmol·mol<sup>−1</sup> CO<sub>2</sub> conditions.
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