Boosting Polyamines to Enhance Shoot Regeneration in Potato (<i>Solanum tuberosum</i> L.) Using AgNO<sub>3</sub>
Walaa M. R. M. Adly | Yasser S. A. Mazrou | Mohammad E. EL-Denary | Mahasen A. Mohamed | El-Sayed T. Abd El-Salam | Ahmed S. Fouad
Advancements in shoot regeneration systems support biotechnology-based tools used in the genetic improvement of plant crops. This study aims to enhance shoot regeneration in potatoes by boosting polyamine content by adding AgNO<sub>3</sub> to the shoot regeneration medium (MS medium supplemented with 30 g L<sup>−1</sup> sucrose, 100 mg L<sup>−1</sup> myoinositol, and 2.25 BA mg L<sup>−1</sup>). Five concentrations of AgNO<sub>3</sub> (2, 4, 6, 8, and 10 mg L<sup>−1</sup>) were used in addition to a control. The effect of AgNO<sub>3</sub> on regeneration assumed a more or less concentration-dependent bell-shaped curve peaking at 4 mg L<sup>−1</sup>. Enhancements in shoot regeneration were attributed to the known role of AgNO<sub>3</sub> as an ethylene action blocker in addition to improvements in polyamine accumulation without an increase in H<sub>2</sub>O<sub>2</sub> content, lipid peroxidation, or DNA damage. The uncoupling of shoot regeneration and polyamine content recorded at high AgNO<sub>3</sub> concentrations can be attributed to the consumption of polyamines to counteract the synchronized oxidative stress manifested by increases in H<sub>2</sub>O<sub>2</sub> content, lipid peroxidation, and DNA damage.
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