Phytochemical Screening and Antibacterial Activity of <i>Taxus baccata</i> L. against <i>Pectobacterium</i> spp. and <i>Dickeya chrysanthemi</i>

The yew tree (<i>Taxus baccata</i> L.) is considered in folklore a symbol of immortality due to its qualities of longevity and regeneration. Despite its poisonous reputation, the yew tree has a long history of medicinal use, particularly in the form of extracts from its leaves and bark. In the work presented herein, gas chromatography–mass spectrometry (GC–MS) chemical profiling was applied to the aqueous ammonia/hydromethanolic extracts of several plant organs of <i>T. baccata</i>, leading to the identification of different bioactive compounds than those previously characterized by high-performance liquid chromatography with tandem mass spectrometry (HPLC–MS/MS) in other extraction media. The leaf aqueous ammonia extract was rich in 2-hexylthiophene and 3-<i>O</i>-methyl-<span style="font-variant: small-caps;">d</span>-fructose; 9-octadecenoic and hexadecanoic acid were the main constituents of the bark aqueous ammonia extract; and the fruit hydromethanolic extract contained methyl 2-<i>O</i>-methyl-α-<span style="font-variant: small-caps;">d</span>-xylofuranoside, 1,3-dioxolane derivatives, and erysimoside. The antimicrobial activity of the extracts was assayed against four bacterial pathogens responsible for the soft rot and blackleg diseases of potatoes, viz. <i>Pectobacterium carotovorum</i> subsp. <i>carotovorum</i>, <i>Pectobacterium atrosepticum</i>, <i>Pectobacterium parmentieri</i>, and <i>Dickeya chrysanthemi</i>, resulting in minimum inhibitory concentration (MIC) values as low as 187 μg·mL⁻¹. Bioassays on potato slices confirmed the efficacy of the leaf extract at this dose when applied as a preventive treatment before artificial inoculation with <i>P. carotovorum</i> subsp. <i>carotovorum</i>. In view of this high activity, these extracts may find application in the integrated pest management of soft rot <i>Pectobacteriaceae</i> (SRP) diseases.