Field resistance of six cultivars of winter oilseed rape against Turnip yellow mosaic virus
Josef Špak | Darina Kubelková
The resistance of six cultivars of winter oilseed rape (SL 509, SL 507, Darmor, Solida, Jet Neuf, Silesia) against Turnip yellow mosaic virus was studied. The number of over-wintering plants and plants with symptoms of TYMV infection were monitored. All plants were tested by the double diffusion test in agar and by DAS-ELISA to prove infection. ELISA was the most sensitive method, revealing 32-76% of latent virus infections in individual cultivars. The results clearly illustrated that monitoring of plant symptoms is not sufficient to prove TYMV infection in the field and that sensitive, large scale methods like ELISA must be employed to obtain reliable data.
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