Evaluation of phenolic content, total flavonoid and survey of antioxidant activity of leaves of Ficus carica and Pterocarya fraxinifolia trees using spectrophotometry and high performance liquid chromatograph methods
2015
Naser Jafari | Pourandokht Naderi | Mohammad Ali Ebrahimzadeh
In this research, to evaluate the antioxidant activity of leaf Pterocarya fraxinifolia (Juglandaceae) and Ficus carica (Moraceae) extract were carried out by spectrophotometry and high performance liquid chromatography methods. The leaves of P. fraxinifolia and F. carica were collected from Whitney and Shast Kalate (Golestan), Noor (Mazandaran) and Asalem (Guilan) forests in Iran. Methanolc extract was used in different experiments. The phenolic compounds (gallic acid, coumaric acid and quercetin) were also measured by using high performance liquid chromatography (HPLC) method.The maximum IC50 for DPPH radical-scavenging activity (595.12±21.4 μg ml-1) were observed in P. fraxinifolia leaves. According to the inhibition time, phenolic compound (gallic acid, coumaric acid and quercetin) in F. carica leaves and gallic acid and coumaric acid were detected of Pterocarya leaves methanol extracts. The maximum amount of gallic acid (78.93) and coumaric acid (8.14) in extracts Pterocarya leaves Asalem and the lowest gallic acid (8.56) and coumaric acid (0.89) milligrams per gram was observed in Ficus leaf of Noor forest. Based on the standard chromatogram retention time of gallic acid (2.383), coumaric acid (3.817) and quercetin (7.217) mg/g was reported. This study showed that soil factors, such as potassium, sodium, phosphorus and nitrogen compounds with antioxidant phenolic extracts of the leaves of both plants there is a significant correlation.
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