Genomic characteristics of classical swine fever virus strains of bovine origin according to primary and secondary sequence structure analysis
Massimo Giangaspero | Shu-Qin Zhang
Background: Classical swine fever virus (CSFV), species member of the family Flaviviridae, is generally considered restricted to domestic and wild suids. Circulation of CFSV has been detected in cattle herds in China and India. Natural infection appeared associated with clinical signs in some cases. Aim: Secondary structures of the internal ribosome entry site (IRES) in the 5’ untranslated region (UTR) were used for genomic characterization of bovine strains. Methods: Sequences have been compared to representative CSFV strains isolated from pigs, vaccines and contaminants from porcine cell lines and an ovine strain isolated in Spain. Results: Observed sequences from cattle showed genetic relatedness with live attenuated vaccine strains used in pigs. Sequence characteristics of the Chinese strain S171 resulted genetically distant from previously reported CSFV genotypes, suggesting a new outgroup in the species, described for the first time and named CSFV-d. Other Chinese strains were genetically closely related to CSFV genotype a2 (Alfort type) pig strains. Indian strains, reported from the states of Tamil Nadu and Meghalaya, were genetically closely related to CSFV genotype a1 (Brescia type) and a5 pig strains, respectively. Conclusion: These preliminary observations are new and relevant in countries where CSFV control and eradication strategies are applied.
Показать больше [+] Меньше [-]Ключевые слова АГРОВОК
Библиографическая информация
Эту запись предоставил Directory of Open Access Journals