In Silico Screening and Development of Microsatellite Markers for Genetic Analysis in <i>Perca fluviatilis</i>
Pu Xu | Cuiyun Lu | Zhipeng Sun | Youyi Kuang | Dingchen Cao | Tangbin Huo | Chao Li | Hongyu Jin | Xianhu Zheng
<i>Perca fluviatilis</i> is an economically important species of freshwater fish. To understand the genetic structure of <i>P. fluviatilis</i> in China, 268 samples were collected from Wulungu Lake (WL), Jili Lake (JL), the Wulungu River (WR), and the Kalaeerqisi River (KR). These samples were then analyzed using microsatellite markers. A total of 98,425 microsatellite markers were developed based on the genomic data, and 29 polymorphic microsatellite markers were selected to analyze genetic diversity in this study. The number of alleles (<i>N<sub>a</sub></i>) and observed heterozygosity (<i>H<sub>o</sub></i>) per population ranged from 4.621 (KR) to 11.172 (WL) and from 0.510 (KR) to 0.716 (JL), respectively. The results of the polymorphic information content (<i>PIC</i>) showed that the WL, JL, and WR populations were highly polymorphic (<i>PIC</i><i>≥</i> 0.5) and that the KR population was moderately polymorphic (0.25 ≤ <i>PIC</i> < 0.5). The genetic differentiation coefficient (<i>F<sub>st</sub></i>) among the four <i>P. fluviatilis</i> populations was 0.074, indicating moderate genetic differentiation among the populations in Xinjiang. The reason for the significant difference between the rivers and lakes could be the presence of a dam blocking the flow of <i>P. fluviatilis</i>. The development of microsatellite markers provides support for population genetics in the future. The evaluation of the genetic structure of <i>P. fluviatilis</i> in Xinjiang provides a reference for the reproduction and conservation of <i>P. fluviatilis</i>.
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