Anaerobic bacterial isolation with histopatholgical exam of liver abscesses in cattle, sheep, and camels in Al-Qadisiyah province

The study was conducted to the isolation of anaerobic bacteria and histopathological examination of liver abscesses. Seventy-five liver abscesses from cattle (30 samples), sheep (35 samples) and camel (10 samples) at the period extended from October 2015 to March 2016, were collected from the slaughterhouse in Al-Qadisiyah province in Iraq. The percentages of macroscopic examination included (70% and 90%) in cattle and camel respectively, and less in sheep (45.71%) of single liver abscess while multi-abscesses reveal higher percentage (54.29%) in sheep than in cattle and camel. The abscesses swabs were cultured for anaerobic bacteria and the following seven species of bacteria were identified: Fusobacterium necropherum (34.78%),(13.64%), Clostridium novyi (21.74%),(31.82%), Cl. chauvoei (4.35%),(13.64%), Eubacterium lentum (13.04%),(4.54%), Cl. inoculum (8.7%),(9.09%), Cl. sordellii (13.04%),(18.18%) and Propionibacterium arabinosum (4.35%),(9.09%) in cattle and sheep respectively and F. necropherum (50%), Cl. chauvoei (25%) and Cl. sordellii (25%) in Camel. In cattle, histopathological examination of liver abscess in cattle infected with F. necropherum and Cl. novyi reveal the presence of caseous necrosis, calcification, inflammatory cells, fibrous connective tissue, and vacuolation of hepatocytes with Langhans giant cell. In sheep, the lesion of liver abscess infected with Cl. novyi reveal severe hemorrhage, dilation of sinusoids, proliferation of kupffer cells, accumulation of infiltrated inflammatory cells, and with Propionibacterium arabinosum included presence of granulomatous lesion with caseous necrosis, calcification, and cellular debris in the center of lesion, inflammatory cells, the lesion surrounding with fibrous connective tissue. In camel, infected with Cl. chauvoei contain the presence of purulent lesion with cellular debris, fibrosis and infiltration of inflammatory cells and liver abscess infected by Cl. sordellii high proliferation and hyperplasia of the bile duct and dilation of sinusoids with a proliferation of kupffer cells.