MULTI DRUG RESISTANCE PROFILE AND ANTIBIOFILM ACTIVITY OF GARLIC OIL ON STAPHYLOCOCCUS AUREUS ISOLATED FROM MILK OF CATTLE SUFFERING WITH MASTITIS
2024
Durgesh Mishra | Arpita Shrivastav | Neeraj Shrivastava | Nitesh Kumar | Swatantra Kumar Singh | Rajeev Ranjan | Namrata Upadhyay
The present study was undertaken on 164 bovine milk samples (cow = 113 and Buffalo = 51) collected from various dairy farms in and around Rewa (Madhya Pradesh, India) and only 24 samples (6.83%) were found to be positive for mastitis when screened through California Mastitis Test (CMT). The prevalence of subclinical mastitis in cattle was 21%. All 17 isolates were phenotypically characterized by mannitol fermentation, biochemical tests (acetoin production by Voges-Proskauer test), beta-galactosidase test susceptibility to novobiocin (5 µ µµg disk), resistance to polymyxin B (300 µg disk). We also observed the biofilm formation ability of all the Staphylococcus aureus strains (n=17) by Congo Red Agar (CRA), Microtiter plate (using crystal violet), and light microscopy method, 90% sensitivity was seen through microtiter plate method. Antibiofilm activity of garlic oil was undertaken on positive isolates (3%, 2%, and 1% concentration) along with positive and negative control in every microtiter plate assay. Maximum inhibition was observed at 3% concentration with O.D values ranging from 0.021±0.007 to 0.291 ± 0.005 in all the samples with percent inhibition (50 to 60%). Multi-drug resistance profiles of biofilm-producing isolates were also undertaken against polymyxin, novobiocin, tetracyclines, cotrimoxazole, clindamycin, cefoxitin, and cefoperazone. Eighty-two (82) percent of isolates showed resistance against polymyxin, 52% against novobiocin and erythromycin, and all the isolates showed 100% sensitivity against tetracyclines and cefoxitin.
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