Analysis of ABA-mediated metabolome response in dehydrated branches of Salix matsudana
2024
HU Yaofang | LI Ao | YIN Jiahui | WANG Yuancheng | LIU Junxiang | SUN Zhenyuan
[Objective] ABA is a key hormone for drought stress in plants. Differential metabolites and their associated pathways of ABA-mediated dehydration response in the isolated branches of Salix matsudana were investigated by metabolomics analysis, which provided the basis for the response to drought stress in S. matsudana. [Methods] The authors investigated the differential response of the xylem metabolomes of the S. matsudana branches dehydrated to different water potentials after soaked in the sodium tungstate solution, an inhibitor of ABA synthesis, using the isolated branches of S. matsudana as materials. [Results] Compared with the deionized water-soaked non-dehydrated branches, in the dehydrated to the P50 water potential branches the endogenous ABA content was significantly increased by 425.032%, and content of the soluble sugars such as D-fructose was significantly increased along with the content of amino acids such as L-glutamic acid. When ABA synthesis inhibitor-soaked branches was compared to deionized water-soaked branches, the content of endogenous ABA was significantly decreased by 79.253%, and the content of the soluble sugars such as D-1, 5-defructose and amino acids such as phenylalanine were significantly reduced after soaked in the inhibitor of ABA synthesis. Plant hormone signal transduction, carotenoid biosynthesis and starch and sucrose metabolism pathways were the key pathways in the dehydrated response of S. matsudana branches. [Conclusion] In the S. matsudana branches after dehydration, the ABA content is increased significantly, mediating the polysaccharide hydrolysis process and other processes to reduce the osmotic potential in response to drought stress.
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