Cysteamine dioxygenase as enzymes for taurine synthesis and the negative effect of high dietary cysteamine on growth and body shape of the common carp, Cyprinus carpio
2019
Gonzales-Plasus, M.M. ((Western Philippines University - Puerto Princesa Campus, Puerto Princesa City, Palawan (Philippines). College of Fisheries and Aquatic Sciences), (Tokyo University of Marine Science and Technology, Minato, Tokyo (Japan). Department of Marine Biosciences)) | Kondo, H. | Hirono, I. | Satoh, S. | Haga, Y.
This study investigated effects of dietary sulfur amino acid-related compounds on the content of these compounds in the hepatopancreas, the gene expression of taurine-synthetic enzymes, somatostatin, and growth hormone, and the growth and morphology of the common carp, Cyprinus carpio. Juvenile common carp were fed one of nine diets: a basal diet supplemented without sulfur compound (control); a basal diet supplemented with 1.0% or 1.5% cysteamine hydrochloride (CSH); 1.5% or 3.0% cysteine; 1.0% or 1.5% methionine; and 0.5% or 1.0% taurine for 30 d. The 1.0% and 1.5% CSH supplementation caused growth retardation and deformities in the fish. All treatments increased carcass taurine levels (18.5-86.9 g/kg). The highest whole body taurine content was observed in fish fed the 1.5% CSH-supplemented diet. Cysteine dioxygenase was downregulated by cysteine and 0.5% taurine but upregulated by 1.5% CSH. Cysteine sulfinic acid decarboxylase was downregulated by cysteine, methionine, and CSH. Cysteamine dioxygenase was downregulated by methionine, cysteine, and 0.5% taurine but upregulated by CSH. Somatostatin 14 was upregulated by CSH. Insulin-like growth factor-1 was upregulated by 1% taurine and cysteine but downregulated by 1.5% CSH. The present study suggests that the cysteamine pathway is mainly responsible for taurine synthesis in common carp.
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