The isolation of infectious African swine fever viruses from pork products seized at Japanese animal quarantine stations
2019
Masujin, K. | Yamazoe, R. | Kameyama, K. | Fujisawa, N. | Kobayashi, Y. | Iwata, K. | Senba, H. | Yamada, M. | Endo, A. | Kokuho, T. | Yanagisawa, M. | Yamakawa, M.
During the period between August 2018 and March 2019 Animal Quarantine Service (AQS) of Japan detected 18 cases of African swine fever virus (ASFV)-specific PCR positive pork products which were voluntarily disclaimed by foreign tourists at the border control. We attempted to isolate ASFV from nine out of eighteen PCR positive products, which were evaluated to be heat-untreated by the catalase activity test, in in vitro cell cultures of primary porcine alveolar macrophages (PAM). We successfully recovered two isolates of ASFV, which were classified in the same genotype (genotype II) with other strains currently circulating in Europe and China based on the nucleotide sequence similarity of the p72 gene. Our findings indicate that infectious ASFV has now been reaching the border via contaminated pork and pork products carried by the tourists from endemic countries and increasing the risk of the occurrence of ASF in Japan.
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